Product nameAnti-Thioredoxin / TRX antibody
See all Thioredoxin / TRX primary antibodies
DescriptionRabbit polyclonal to Thioredoxin / TRX
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- HeLa and MCF7 cell lysates; Human liver cancer tissue lysate; Human thyroid cancer tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/2000 - 1/10000.|
|WB||1/600 - 1/5000. Predicted molecular weight: 12 kDa.|
|IHC-P||1/40 - 1/200.|
FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Sequence similaritiesBelongs to the thioredoxin family.
Contains 1 thioredoxin domain.
modificationsIn the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
- Information by UniProt
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
All lanes : Anti-Thioredoxin / TRX antibody (ab185329) at 1/600 dilution
Lane 1 : Hela cell tissue lysate
Lane 2 : MCF7 cell tissue lysate
Lane 3 : Human liver cancer tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 12 kDa
Exposure time: 20 seconds
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Thioredoxin / TRX using ab185329 at a 1/40 dilution.
ab185329 has not yet been referenced specifically in any publications.