Overview

  • Product name

  • Description

    Rabbit polyclonal to THOC2/Tho2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human THOC2/Tho2 aa 1543-1593.
    Database link: NP_001075019.1

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899)
  • General notes

     This product was previously labelled as THOC2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab129485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 183 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex.The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
  • Sequence similarities

    Belongs to the THOC2 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CXorf3 antibody
    • DJ506G2.1 antibody
    • hTREX120 antibody
    • RP5 1189B24.5 antibody
    • THO complex 2 antibody
    • THO complex subunit 2 antibody
    • Tho2 antibody
    • THOC2 antibody
    • THOC2_HUMAN antibody
    see all

Images

  • All lanes : Anti-THOC2/Tho2 antibody (ab129485) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling THOC2/Tho2 with ab129485 at 1/1000 (1µg/mg). Detection: DAB.

  • Lane 1: ab129485 at 1 µg/ml staining THOC2/Tho2 in HeLa whole cell lysate immunoprecipitated using ab129485 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.
    Detection: Chemiluminescence
    Exposure time: 1 second

References

This product has been referenced in:

  • Hjorth-Jensen K  et al. SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability. Nucleic Acids Res 46:9484-9495 (2018). Read more (PubMed: 30124983) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 01 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Tris-Glycine)
Loading amount
40 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 29 2016

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