Product nameAnti-THOC5 antibody
See all THOC5 primary antibodies
DescriptionRabbit polyclonal to THOC5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between amino acids 300 and 325 of Human THOC5 (NP_003669.4)
- Hela, 293T or NIH3T3 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86070 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 79 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the THOC5 family.
modificationsPhosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- C22orf19 antibody
- Chromosome 22 open reading frame 19 antibody
- Fmip antibody
All lanes : Anti-THOC5 antibody (ab86070) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 90 seconds
Detection of THOC5 by Western blot of Immunprecipitate.
ab86070, at 0.4 µg/ml, staining THOC5 in HeLa whole cell lysate immunoprecipitated using ab86070 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Szczesny RJ et al. Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system. PLoS One 13:e0194887 (2018). Read more (PubMed: 29590189) »
- Li M et al. RECQ5-dependent SUMOylation of DNA topoisomerase I prevents transcription-associated genome instability. Nat Commun 6:6720 (2015). Read more (PubMed: 25851487) »