Overview

  • Product name

    Anti-THOC5 antibody [EP6904]
    See all THOC5 primary antibodies
  • Description

    Rabbit monoclonal [EP6904] to THOC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICCmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids in Human THOC5 (UniProt ID: Q13769).

  • Positive control

    • 293T and HeLa cell lysates; 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP6904
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the THOC5 family.
    • Post-translational
      modifications

      Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • C22orf19 antibody
      • Chromosome 22 open reading frame 19 antibody
      • Fmip antibody
      • Fms interacting protein antibody
      • fSAP79 antibody
      • Functional spliceosome-associated protein 79 antibody
      • hTREX90 antibody
      • KIAA0983 antibody
      • LOC400922 antibody
      • NF2/meningioma region protein pK1.3 antibody
      • PK1.3 antibody
      • Placental protein 39.2 antibody
      • PP39.2 antibody
      • THO complex 5 antibody
      • THO complex subunit 5 antibody
      • THO complex subunit 5 homolog antibody
      • thoc5 antibody
      • THOC5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-THOC5 antibody [EP6904] (ab137051) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC-12

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution


      Predicted band size: 79 kDa

    • Flow cytometric analysis of permeabilized 293T cells labelling THOC5 with ab137051 at dilution 1/10 (red) or a Rabbit IgG negative control (green).

    References

    ab137051 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137051.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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