Product nameAnti-THOC7 antibody
See all THOC7 primary antibodies
DescriptionRabbit polyclonal to THOC7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Fusion protein corresponding to Human THOC7 aa 1-204. (BC065012; The identity of the protein fusion partner is GST).
MGAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFIKWCNSGSQEEGYSQY QRMLSTLSQCEFSMGKTLLVYDMNLREMENYEKIYKEIECSIAGAHEKIA ECKKQILQAKRIRKNRQEYDALAKVIQHHPDRHETLKELEALGKELEHLS HIKESVEDKLELRRKQFHVLLSTIHELQQTLENDEKLSEVEEAQEASMET DPKP
Database link: Q6I9Y2
- Mouse spleen, Human fetal liver and Mouse testis tissue lysates; HeLa and A431 cell lysates; Human lung cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab199150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 24 kDa.|
FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.
Sequence similaritiesBelongs to the THOC7 family.
Cellular localizationCytoplasm. Nucleus. Interaction with THOC5 is required for nuclear localization.
- Information by UniProt
- FLJ23445 antibody
- fSAP24 antibody
- Functional spliceosome associated protein 24 antibody
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling THOC7 with ab199150 at 1/30 dilution.
All lanes : Anti-THOC7 antibody (ab199150) at 1/500 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HeLa cell lysate
Lane 4 : Mouse testis lysate
Lane 5 : A431 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 24 kDa
Exposure time: 15 seconds
Gel: 10% SDS-PAGE.
ab199150 has not yet been referenced specifically in any publications.