Overview

  • Product name

  • Description

    Rabbit polyclonal to THOC7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human THOC7 aa 154-204. The exact sequence is proprietary.
    Database link: Q6I9Y2

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to THOC7 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.
  • Sequence similarities

    Belongs to the THOC7 family.
  • Cellular localization

    Cytoplasm. Nucleus. Interaction with THOC5 is required for nuclear localization.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ23445 antibody
    • fSAP24 antibody
    • Functional spliceosome associated protein 24 antibody
    • Functional spliceosome-associated protein 24 antibody
    • hTREX30 antibody
    • Ngg1 interacting factor 3 like 1 binding protein 1 antibody
    • Ngg1 interacting factor 3 like protein 1 binding protein 1 antibody
    • Ngg1-interacting factor 3-like protein 1-binding protein 1 antibody
    • NIF3L1 binding protein 1 antibody
    • NIF3L1-binding protein 1 antibody
    • NIF3L1BP1 antibody
    • THO complex 7 homolog (Drosophila) antibody
    • THO complex 7 homolog antibody
    • THO complex subunit 7 homolog antibody
    • THOC 7 antibody
    • thoc7 antibody
    • THOC7_HUMAN antibody
    see all

Images

  • All lanes : Anti-THOC7 antibody (ab241345) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • THOC7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241345 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241345 at 0.4 µg/ml.

    Lane 1: ab241345 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

References

ab241345 has not yet been referenced specifically in any publications.

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