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    thomsen-friedenreich-antigen-antibody-spm320-prediluted-ab17785.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

  • Datasheet
Submit a review Q&A (1)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

    Key features and details

    • Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgM

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    Overview

    • Product name

      Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted
      See all Thomsen-Friedenreich Antigen primary antibodies
    • Description

      Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Tissue, cells or virus corresponding to Human Thomsen-Friedenreich Antigen.

    • Epitope

      Galß1-3GalNAc
    • Positive control

      • KG1 cells. Human colorectal carcinoma tissues.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.2
      Preservative: 0.09% Sodium azide
      Constituents: Tris buffered saline, 1% BSA
    • Concentration information loading...
    • Purity

      Purified IgM
    • Clonality

      Monoclonal
    • Clone number

      SPM320
    • Isotype

      IgM
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Isotype control

      • Mouse IgM [B11/7] - Isotype control (ab91545)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17785 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Relevance

      The Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.
    • Cellular localization

      Cell Membrane
    • Alternative names

      • CD176 antibody
      • TF antibody
      • TF antigen antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)
      Human colon adenocarcinoma stained with ab17785 (prediluted)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab17785? Please let us know so that we can cite the reference in this datasheet.

    ab17785 has been referenced in 3 publications.

    • Saha S  et al. Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways. Biochem Biophys Rep 5:313-327 (2016). PubMed: 28955838
    • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . PubMed: 24586486
    • Kumagai H  et al. Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer. Int J Cancer : (2012). Human . PubMed: 23055136

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    what solution is the Ab in?
    might try in WB

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2013

    Answer

    ab17785 is supplied in a TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

    Read More

    Abcam Scientific Support

    Answered on May 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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