Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

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Key features and details

  • Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted
    See all Thomsen-Friedenreich Antigen primary antibodies

  • Description

    Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Tissue/ cell preparation (Human). Neuraminidase-treated human red blood cells.

  • Epitope

    Galß1-3GalNAc

  • Positive control

    • KG1 cells. Human colorectal carcinoma tissues.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.60
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 1% BSA
  • Concentration information loading...

  • Purity

    Purified IgM

  • Clonality

    Monoclonal

  • Clone number

    SPM320

  • Isotype

    IgM

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    The Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.

  • Cellular localization

    Cell Membrane

  • Alternative names

    • CD176 antibody
    • TF antibody
    • TF antigen antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)
    Human colon adenocarcinoma stained with ab17785 (prediluted)

References

Publishing research using ab17785? Please let us know so that we can cite the reference in this datasheet.

ab17785 has been referenced in 3 publications.

  • Saha S  et al. Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways. Biochem Biophys Rep 5:313-327 (2016). PubMed: 28955838
  • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . PubMed: 24586486
  • Kumagai H  et al. Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer. Int J Cancer : (2012). Human . PubMed: 23055136

Customer reviews and Q&As

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Question

what solution is the Ab in?
might try in WB

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Answer

ab17785 is supplied in a TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

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