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  1. Link

    thp-1-whole-cell-lysate-ab7913.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human Other
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THP-1 whole cell lysate (ab7913)

  • Datasheet
  • SDS
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Overview

  • Product name

    THP-1 whole cell lysate
    See all THP1 lysates
  • General notes

    Cell line: THP-1 (Human acute monocytic leukemia).
    Growth media: RPMI and 10% FBS.

    THP-1 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 0.77% DTT.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituents: 0.1% Sodium lauryl sulfate, 0.02% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.01% Aprotinin, 0.01% Leupeptin, 0.1% Triton X-100, 0.1% PMSF, 0.5% Tris(2,2'-bipyridyl)ruthenium(II) Chloride Hexahydrate, 0.2% 1-(4-Chloro-2,5-dimethoxyphenyl)-2-aminopropaneHCL , 8.96% Water
  • Concentration information loading...
  • Lysate notes

    THP-1 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 0.77% DTT.

  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • Other
  • Background

    Properties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
THP-1 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Notes
WB
Use at an assay dependent concentration.

THP-1 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab7913? Please let us know so that we can cite the reference in this datasheet.

ab7913 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Is Cyclooxygaenase-2 detectable in the THP-1 lysate?

Read More

Abcam community

Verified customer

Asked on Sep 19 2002

Answer

Thank you for your inquiry. We have not tested Cyclooxygaenase-2 in our THP-1 lysate.

Read More

Abcam Scientific Support

Answered on Sep 20 2002

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