Product nameTHP1 nuclear extract lysate (PMA stimulated)
See all THP1 lysates
DescriptionTHP1 nuclear extract lysate
General notesCell line: THP1 (Human acute monocytic leukemia).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
The protein content has been determined by a Bradford-based assay.
The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
Note: With Protease inhibitors.
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BackgroundProperties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.
Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.
ab14901 has not yet been referenced specifically in any publications.