THP1 nuclear extract lysate (PMA stimulated) (ab14901)


  • Product name
    THP1 nuclear extract lysate (PMA stimulated)
    See all THP1 lysates
  • Description
    THP1 nuclear extract lysate
  • General notes
    Cell line: THP1 (Human acute monocytic leukemia).
    To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
    The protein content has been determined by a Bradford-based assay.
    The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).


  • Form
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors.
  • Concentration information loading...
  • Research areas
  • Background
    Properties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.


  • Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.


ab14901 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your enquiry. For the stimulation of THP1 cells with PMA please perform the following; Grow THP-1 cells at a density range of 5-8 x 10exp5 c/ml., add PMA to a final concentration of 10 ng/ml to stimulate the cells. Do not add anything to unstimulated cells. Incubate for 24 hrs. After 24 hrs, most of the stimulated cells should be attached to the flask. Then harvest the cells in ice-cold PBS buffer containing phosphatases inhibitors. Remove the PBS and lyse the cells by osmotic shock using a hypotonic buffer. Collect the nuclei and lyse them with lysis buffer (high salt buffer containing proteases inhibitors). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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