Overview

  • Product name

    Anti-THRAP5 antibody
  • Description

    Rabbit polyclonal to THRAP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Turkey, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 550-600 of Human THRAP5 (NP_005472.2).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab130996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 16 family.
    Contains 5 WD repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DRIP 92 antibody
    • DRIP92 antibody
    • MED 16 antibody
    • MED16 antibody
    • MED16_HUMAN antibody
    • Mediator complex subunit 16 antibody
    • Mediator of RNA polymerase II transcription subunit 16 antibody
    • THRAP 5 antibody
    • Thyroid hormone receptor associated protein 5 antibody
    • Thyroid hormone receptor associated protein 95 kD subunit antibody
    • Thyroid hormone receptor associated protein complex 95 kDa component antibody
    • Thyroid hormone receptor-associated protein 5 antibody
    • Thyroid hormone receptor-associated protein complex 95 kDa component antibody
    • Trap 95 antibody
    • Trap95 antibody
    • Vitamin D3 receptor interacting protein complex component antibody
    • Vitamin D3 receptor-interacting protein complex 92 kDa component antibody
    see all

Images

  • All lanes : Anti-THRAP5 antibody (ab130996) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 3 minutes
  • Detection of THRAP5 by Western Blot of Immunprecipitate.

    ab130996 at 1µg/ml staining THRAP5 in HeLa whole cell lysate immunoprecipitated using ab130996 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure times of 30 seconds

References

This product has been referenced in:

  • Kobayashi EH  et al. Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription. Nat Commun 7:11624 (2016). IP . Read more (PubMed: 27211851) »
  • Sekine H  et al. The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression. Mol Cell Biol 36:407-20 (2015). Read more (PubMed: 26572828) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - nuclear (293)
Loading amount
10 µg
Specification
293
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up