Product nameAnti-THRAP5 antibody
DescriptionRabbit polyclonal to THRAP5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Turkey, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between amino acids 550-600 of Human THRAP5 (NP_005472.2).
- HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab130996 is affinity purified using an epitope specific to THRAP5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab130996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 97 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 16 family.
Contains 5 WD repeats.
- Information by UniProt
- DRIP 92 antibody
- DRIP92 antibody
- MED 16 antibody
All lanes : Anti-THRAP5 antibody (ab130996) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 3 minutes
Detection of THRAP5 by Western Blot of Immunprecipitate.
ab130996 at 1µg/ml staining THRAP5 in HeLa whole cell lysate immunoprecipitated using ab130996 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure times of 30 seconds
This product has been referenced in:
- Kobayashi EH et al. Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription. Nat Commun 7:11624 (2016). IP . Read more (PubMed: 27211851) »
- Sekine H et al. The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression. Mol Cell Biol 36:407-20 (2015). Read more (PubMed: 26572828) »