Overview

  • Product name

    Thrombin activity assay
    See all Thrombin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    0.035 AU/ml
  • Range

    0.05 AU/ml - 1.6 AU/ml
  • Product overview

    Thrombin Activity Assay Kit (ab234620) is developed to determine thrombin activity in human plasma, serum and cell culture samples. The amidolytic activity of thrombin is quantitated using a highly specific thrombin substrate releasing a pNA chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the thrombin enzymatic activity.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Thrombin (activated factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease (EC 3.4.21.5) that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin that is the immediate end product of prothrombin activation: two further thrombin products can be identified, beta- and gamma- thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    EIA Diluent Concentrate (10x) 1 x 20ml
    Human Thrombin Standard 1 vial
    Microplate 1 unit
    Sealing Tapes 3 units
    Thrombin Substrate 2 vials
  • Research areas

  • Function

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Alternative names

    • Coagulation factor II
    • Coagulation factor II thrombin
    • EC 3.4.21.5
    • F2
    • Factor II
    • Prepro coagulation factor II
    • Prothrombin
    • Prothrombin B chain
    • PT
    • RPRGL2
    • Serine protease
    • THPH1
    • THRB_HUMAN
    • Thrombin heavy chain
    see all

Images

Protocols

References

ab234620 has not yet been referenced specifically in any publications.

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