Product nameThrombin Activity Assay Kit (Fluorometric)
See all Thrombin kits
Sample typePlasma, Purified protein
Species reactivityReacts with: Mammals, Other species
Thrombin Activity Assay Kit (Fluorometric) (ab197006) is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
In the thrombin activity assay protocol, a synthetic AMC-based peptide substrate is proteolytically cleaved by thrombin to release a fluorophore, AMC, which can be easily quantified by fluorescence reader at Ex/Em = 350/450 nm.
Thrombin activity assay protocol summary:
- add standards and samples to wells
- add reaction mix
- analyze with a microplate reader for 30-60 min
Thrombin enzyme (Factor IIa, EC 184.108.40.206), a serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Thrombin Assay Buffer 1 x 15ml Thrombin Dilution Buffer 1 x 1ml Thrombin Enzyme Standard 1 x 5µl Thrombin Substrate 1 x 0.5ml
FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseFactor II deficiency
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- Coagulation factor II
- Prepro coagulation factor II
This product has been referenced in:
- Shefa AA et al. Enhancement of hemostatic property of plant derived oxidized nanocellulose-silk fibroin based scaffolds by thrombin loading. Carbohydr Polym 208:168-179 (2019). Read more (PubMed: 30658788) »
- He P et al. Timely and large dose of clotting factor IX provides better joint wound healing after hemarthrosis in hemophilia B mice. Int J Hematol 110:59-68 (2019). Read more (PubMed: 31006077) »