Overview

  • Product name

  • Description

    Sheep polyclonal to Thrombin
  • Host species

    Sheep
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein purified Human Thrombin

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab61367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Coagulation factor II antibody
    • Coagulation factor II thrombin antibody
    • EC 3.4.21.5 antibody
    • F2 antibody
    • Factor II antibody
    • Prepro coagulation factor II antibody
    • Prothrombin antibody
    • Prothrombin B chain antibody
    • PT antibody
    • RPRGL2 antibody
    • Serine protease antibody
    • THPH1 antibody
    • THRB_HUMAN antibody
    • Thrombin heavy chain antibody
    see all

References

ab61367 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your message.

We do not recommend to dilute antibodies before storing them. Antibodies can be unstable when stored at a lower dilution and this can affect their quality. We recommend to store our products as stated on the individual online datasheets to ensure they remain of good quality and that we can continue to provide our Abpromise guarantee.

The vial of antibody should contain about 55 ul of antibody. I can suggest to aliquot in 10 ul or 15 ul.

I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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