• Product name
    Anti-Thrombin antibody [BDI905]
    See all Thrombin primary antibodies
  • Description
    Mouse monoclonal [BDI905] to Thrombin
  • Host species
  • Specificity
    Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (purified) (Human)



Our Abpromise guarantee covers the use of ab20877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Involvement in disease
      Factor II deficiency
      Ischemic stroke
      Thrombophilia due to thrombin defect
      Pregnancy loss, recurrent, 2
    • Sequence similarities
      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • Post-translational
      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
      N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
    • Cellular localization
      Secreted, extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • Prothrombin B chain antibody
      • PT antibody
      • RPRGL2 antibody
      • Serine protease antibody
      • THPH1 antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all


    • ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.


    ab20877 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Since I do not know what application the antibody is used in, I am providing a list of possible secondary antibodies.
    Please choose an antibody that is suitable for the planned application. If you would like some help with this, please let me know which application is used.
    HRP conjugated:
    ab98693 https://www.abcam.com/index.html?datasheet=98693 https://www.abcam.com/index.html?datasheet=98693.
    AP conjugated:
    ab97237 https://www.abcam.com/index.html?datasheet=97237 https://www.abcam.com/index.html?datasheet=97237.
    conjugated to fluorophore:
    ab130786 https://www.abcam.com/index.html?datasheet=130786 https://www.abcam.com/index.html?datasheet=130786.
    ab130785 https://www.abcam.com/index.html?datasheet=130785 https://www.abcam.com/index.html?datasheet=130785.
    ab136127 https://www.abcam.com/index.html?datasheet=136127 https://www.abcam.com/index.html?datasheet=136127.
    ab97239 https://www.abcam.com/index.html?datasheet=97239 https://www.abcam.com/index.html?datasheet=97239.
    ab99605 https://www.abcam.com/index.html?datasheet=99605 https://www.abcam.com/index.html?datasheet=99605.

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    Thank you for your enquiry. The isolelectric points and the percentage of glycosylation have not, to our knowledge,been determined for the three antibodies you asked about (ab35799, ab20626, and, in a separate e-mail, ab20877). These are all IgG1 isotype monoclonals, and the molecular weight of IgG, regardless of isotype,is estimated to be about 150 kDa.

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