Key features and details
- Mouse monoclonal [BDI905] to Thrombin
- Suitable for: ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Thrombin antibody [BDI905]
See all Thrombin primary antibodies
DescriptionMouse monoclonal [BDI905] to Thrombin
SpecificityAb20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
Tested applicationsSuitable for: ICC/IF, ELISAmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Full length protein (purified) (Human)
Storage instructionsAvoid freeze / thaw cycle.
Storage bufferConstituent: 50% Glycerol
Concentration information loading...
Purification notesThe antibody has been purified from tissue culture by Ammonium sulfate precipitation, ion-exchange chromatography and gel filtration.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab20877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseFactor II deficiency
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Coagulation factor II antibody
- Coagulation factor II thrombin antibody
- EC 126.96.36.199 antibody
ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab20877 has been referenced in 1 publication.
- Krämer SD et al. How to copy and paste DNA microarrays. Sci Rep 9:13940 (2019). PubMed: 31558745