Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds, Plasma
Product nameThrombin Inhibitor Screening Assay Kit (Fluorometric)
See all Thrombin kits
Sample typePlasma, Inhibitor compounds
Species reactivityReacts with: Mammals, Other species
Thrombin Inhibitor Screening Assay Kit (Fluorometric) (ab197007) is an assay where the synthetic AMC-based peptide substrate provided is proteolytically cleaved by Thrombin in order to release a fluorophore, AMC, which can be easily quantified by fluorescence reader at Ex/Em = 350/450 nm. In the presence of Thrombin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution.
The kit provides a simple and rapid method to screen potential inhibitors of thrombin and to characterize thrombin inhibitors found in plasma samples.
Thrombin enzyme (Factor IIa) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a serine protease (EC 22.214.171.124) that catalyzes many coagulation-related reactions. Thrombin inhibitors are used as anticoagulants to prevent arterial and venous thrombosis. Some of these inhibitors are currently in clinical use while others are in clinical development.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Thrombin Assay Buffer 1 x 15ml Thrombin Dilution Buffer 1 x 1ml Thrombin Enzyme 1 x 5µl Thrombin Inhibitor 1 x 10µl Thrombin Substrate 1 x 0.5ml
FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseFactor II deficiency
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Coagulation factor II
- Coagulation factor II thrombin
- EC 126.96.36.199
Inhibition of thrombin activity by a thrombin Inhibitor (PPACK Dihydrochloride).
Thrombin activity was measured in plasma samples in the presence and absence of Thrombin Inhibitor (PPACK Dihydrochloride). S = Substrate, I = Inhibitor, AB = Activation Buffer containing Factor Xa. Assays were performed following the kit protocol.
ab197007 has not yet been referenced specifically in any publications.