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Rabbit polyclonal to Thrombin Receptor
ab63445 detects endogenous levels of total Thrombin Receptor protein.
Synthetic peptide corresponding to Human Thrombin Receptor (N terminal).
HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride Without Mg2+ and Ca2+
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Immunogen affinity purified
ab63445 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Use a concentration of 1 - 5 µg/ml.
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
Platelets and vascular endothelial cells.
Belongs to the G-protein coupled receptor 1 family.
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.
Information by UniProt
Coagulation factor II (thrombin) receptor antibody
Coagulation factor II receptor antibody
Western blot - Anti-Thrombin Receptor antibody (ab63445)
All lanes : Anti-Thrombin Receptor antibody (ab63445) at 1/500 dilution Lane 1 : HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours) Lane 2 : HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours) with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 47 kDa Observed band size: 47 kDa Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Thrombin Receptor antibody (ab63445)
ICC/IF image of ab63445 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63445, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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