Overview

  • Product name

    Anti-Thrombomodulin antibody
    See all Thrombomodulin primary antibodies
  • Description

    Rabbit polyclonal to Thrombomodulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide within Human Thrombomodulin aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab106425)

  • Positive control

    • This antibody gave a positive signal in the following Human tissue lysates: Heart; Lung; Kidney; Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab94373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificity

    Endothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in disease

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHUS 6 antibody
    • AHUS6 antibody
    • BDCA 3 antibody
    • BDCA3 antibody
    • CD 141 antibody
    • CD141 antibody
    • CD141 antigen antibody
    • Fetomodulin antibody
    • Thbd antibody
    • THPH12 antibody
    • THRM antibody
    • Thrombomodulin antibody
    • TM antibody
    • TRBM_HUMAN antibody
    see all

Images

  • All lanes : Anti-Thrombomodulin antibody (ab94373) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 21 kDa, 32 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Thrombomodulin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

This product has been referenced in:

  • Araujo TLS  et al. Cell-surface HSP70 associates with thrombomodulin in endothelial cells. Cell Stress Chaperones 24:273-282 (2019). Read more (PubMed: 30645756) »
See 1 Publication for this product

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