Anti-Thrombomodulin antibody [PBS-01] (ab6980)
- Datasheet
- References (11)
- Protocols
Overview
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Product nameAnti-Thrombomodulin antibody [PBS-01]
See all Thrombomodulin primary antibodies -
DescriptionMouse monoclonal [PBS-01] to Thrombomodulin
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Rabbit, Human
Does not react with: Pig -
Immunogen
Recombinant full length protein (Human).
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EpitopeEGF domain 5 of thrombomodulin.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 6.50
Constituents: 0.292% Sodium chloride, 4% Sucrose, 0.1% Tween, 0.41% Sodium acetate -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberPBS-01
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MyelomaSp2
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab6980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionThrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
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Tissue specificityEndothelial cells are unique in synthesizing thrombomodulin.
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Involvement in diseaseDefects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similaritiesContains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
Post-translational
modificationsN-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 7056 Human
- Omim: 188040 Human
- SwissProt: P07204 Human
- Unigene: 2030 Human
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Alternative names
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
Images
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![Western blot - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.com/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-6.jpg)
Western blot - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Image courtesy of an anonymous Abreview.Anti-Thrombomodulin antibody [PBS-01] (ab6980) at 1/100 dilution + whole cell lysate prepared from human aortic endothelial cells at 30 µg
Secondary
HRP conjugated anti-mouse IgG at 1/4000 dilution
Developed using the ECL technique.
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
36kDa band is GAPDH in the same lane. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.com/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.com/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-7.jpg)
Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References
This product has been referenced in:
- Song J et al. Thrombomodulin (TM) in tumor cell differentiation and periphery blood immune microenvironment in oral squamous cell carcinoma. Clin Immunol 191:27-33 (2018). IHC-P ; Human . Read more (PubMed: 29562205) »
- Nunes Dos Santos RM et al. CRISPR/Cas and recombinase-based human-to-pig orthotopic gene exchange for xenotransplantation. J Surg Res 229:28-40 (2018). Read more (PubMed: 29937002) »
