Key features and details
- Mouse monoclonal [Phx-01] to Thrombomodulin
- Suitable for: IHC-Fr, WB, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Thrombomodulin antibody [Phx-01]
See all Thrombomodulin primary antibodies
DescriptionMouse monoclonal [Phx-01] to Thrombomodulin
Tested applicationsSuitable for: IHC-Fr, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mammals
Recombinant full length protein (Human)
EpitopeThis antibody reacts with an epitope within the EGF 5 domain.
ab24595 inhibits thrombin binding.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Thimerosal (merthiolate)
Constituents: 0.09% Tris glycine, PBS
Concentration information loading...
Primary antibody notesab24595 inhibits thrombin binding.
Our Abpromise guarantee covers the use of ab24595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 60 kDa.|
FunctionThrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Tissue specificityEndothelial cells are unique in synthesizing thrombomodulin.
Involvement in diseaseDefects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Sequence similaritiesContains 1 C-type lectin domain.
Contains 6 EGF-like domains.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
Immunohistochemistry (Frzen sections) of human placenta useing ab24595 at 1/100.
All lanes : Anti-Thrombomodulin antibody [Phx-01] (ab24595) at 1 µg/ml
Lane 1 : HL-60 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : HRP Goat anti-Mouse secondary antibody at 1/3000 dilution
Predicted band size: 60 kDa
ab24595 has been referenced in 1 publication.
- Ikezoe T et al. The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective function and prevents SOS in a murine model. Bone Marrow Transplant 52:73-79 (2017). PubMed: 27427915