Overview

  • Product name

    Anti-Thrombomodulin antibody [THBD/1591]
    See all Thrombomodulin primary antibodies
  • Description

    Mouse monoclonal [THBD/1591] to Thrombomodulin
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Thrombomodulin aa 69-194. The exact sequence is proprietary.
    Database link: P07204

  • Positive control

    • Human cervical carcinoma, bladder carcinoma, placenta and angiosarcoma tissues; THP1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab218327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificity

    Endothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in disease

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHUS 6 antibody
    • AHUS6 antibody
    • BDCA 3 antibody
    • BDCA3 antibody
    • CD 141 antibody
    • CD141 antibody
    • CD141 antigen antibody
    • Fetomodulin antibody
    • Thbd antibody
    • THPH12 antibody
    • THRM antibody
    • Thrombomodulin antibody
    • TM antibody
    • TRBM_HUMAN antibody
    see all

Images

  • ab218327 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • All lanes : Anti-Thrombomodulin antibody [THBD/1591] (ab218327) at 1 µg/ml

    Lane 1 : Recombinant Thrombomodulin protein
    Lane 2 : THP1 cell lysate

    Predicted band size: 60 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling Thrombomodulin with ab218327 at 2 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Thrombomodulin with ab218327 at 2 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling Thrombomodulin with ab218327 at 2 µg/ml.

References

ab218327 has not yet been referenced specifically in any publications.

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