Anti-Thrombopoietin antibody [1B11] (ab128487)
Key features and details
- Mouse monoclonal [1B11] to Thrombopoietin
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-Thrombopoietin antibody [1B11]
See all Thrombopoietin primary antibodies -
Description
Mouse monoclonal [1B11] to Thrombopoietin -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Thrombopoietin aa 250-303.
Database link: P40225 -
Positive control
- Human Thrombopoietin (amino acids 250-303) recombinant protein; MCF7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1B11 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
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ELISA |
1/10000.
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Flow Cyt |
1/200 - 1/400.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa. |
ELISA
1/10000. |
Flow Cyt
1/200 - 1/400. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
Sequence similarities
Belongs to the EPO/TPO family. -
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7066 Human
- Omim: 600044 Human
- SwissProt: P40225 Human
- Unigene: 1166 Human
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Alternative names
- C mpl ligand antibody
- C-mpl ligand antibody
- Megakaryocyte colony stimulating factor antibody
see all
Images
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ab128487, at a 1/200 dilution, staining Thrombopoietin in MCF7 cells by Flow Cytometry (green). Negative control is shown in red.
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ELISA using ab128487.
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Anti-Thrombopoietin antibody [1B11] (ab128487) at 1/500 dilution + Human Thrombopoietin (amino acids 250-303) recombinant protein
Predicted band size: 37 kDa
Expected MWt of recombinant protein is 30 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128487 has not yet been referenced specifically in any publications.