For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    thrombopoietin-antibody-1b11-ab128487.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Fibrinolysis / Thrombolysis
Share by email

Anti-Thrombopoietin antibody [1B11] (ab128487)

  • Datasheet
  • SDS
Submit a review Q&A (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-Thrombopoietin antibody [1B11] (ab128487)
  • ELISA - Anti-Thrombopoietin antibody [1B11] (ab128487)
  • Western blot - Anti-Thrombopoietin antibody [1B11] (ab128487)

Key features and details

  • Mouse monoclonal [1B11] to Thrombopoietin
  • Suitable for: WB, ELISA, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
ELISA
Product image
Human Thrombopoietin ELISA Kit (ab219632)

View more associated products

Overview

  • Product name

    Anti-Thrombopoietin antibody [1B11]
    See all Thrombopoietin primary antibodies
  • Description

    Mouse monoclonal [1B11] to Thrombopoietin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human Thrombopoietin aa 250-303.
    Database link: P40225

  • Positive control

    • Human Thrombopoietin (amino acids 250-303) recombinant protein; MCF7 cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1B11
  • Isotype

    IgG2a
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Cancer
    • Cell cycle
    • Cell differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human Thrombopoietin protein (ab9719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • Involvement in disease

    Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • Sequence similarities

    Belongs to the EPO/TPO family.
  • Domain

    Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P40225 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7066 Human
    • Omim: 600044 Human
    • SwissProt: P40225 Human
    • Unigene: 1166 Human
    • Alternative names

      • C mpl ligand antibody
      • C-mpl ligand antibody
      • Megakaryocyte colony stimulating factor antibody
      • Megakaryocyte colony-stimulating factor antibody
      • Megakaryocyte growth and development factor antibody
      • Megakaryocyte stimulating factor antibody
      • MGC163194 antibody
      • MGDF antibody
      • MKCSF antibody
      • ML antibody
      • MPL ligand antibody
      • MPLLG antibody
      • Myeloproliferative leukemia virus oncogene ligand antibody
      • Prepro thrombopoietin antibody
      • THCYT1 antibody
      • THPO antibody
      • Thrombopoietin antibody
      • Thrombopoietin nirs variant 1 antibody
      • TPO antibody
      • TPO_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-Thrombopoietin antibody [1B11] (ab128487)
      Flow Cytometry - Anti-Thrombopoietin antibody [1B11] (ab128487)
      ab128487, at a 1/200 dilution, staining Thrombopoietin in MCF7 cells by Flow Cytometry (green). Negative control is shown in red.
    • ELISA - Anti-Thrombopoietin antibody [1B11] (ab128487)
      ELISA - Anti-Thrombopoietin antibody [1B11] (ab128487)
      ELISA using ab128487.
    • Western blot - Anti-Thrombopoietin antibody [1B11] (ab128487)
      Western blot - Anti-Thrombopoietin antibody [1B11] (ab128487)
      Anti-Thrombopoietin antibody [1B11] (ab128487) at 1/500 dilution + Human Thrombopoietin (amino acids 250-303) recombinant protein

      Predicted band size: 37 kDa



      Expected MWt of recombinant protein is 30 kDa.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab128487? Please let us know so that we can cite the reference in this datasheet.

    ab128487 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    we transafected and transfecting every week cMPL ( Receptor of Thrombopietin ) and put rTHPO into the cell culture to find out the bindung way. But the Problem ist that all the antobodys which we tried from you are not working in this way!

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2012

    Answer

    Thank you for your reply. If I am understanding your assay correctly, your cells are overexpressing a thrombopoietin receptor and you are incubating the cell culture with recombinant thrombopoietin to measure the binding efficacy. What type of recombinant thrombopoietin are you using? It would be very helpful if you could let me know the manufacturer and the catalog number. Have you tried a dot blot or WB with the recombinant protein to see if it is recognized by these antibodies? If you are able to detect the recombinant protein by itsself, there may be an issue with the receptor. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Nov 08 2012

    Question

    my cells are overexpressing a thrombopoietin receptor and we are incubating the cell culture with recombinant thrombopoietin to measure different things.

    Recombinant Human Thrombopoietin
    http://www.rndsystems.com/pdf/288-tp.pdf
    25 µg
    RundD Systems

    I trieted my cells with TPO and i have done a lot of WB with other antibodies and they are all fine,.

    But the antibodies which i bought from you do not function in WB; IF and FACS in my/ our hands.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2012

    Answer

    Thank you for your reply. I would still recommend checking if the antibodies bind to the recombinant protein in a dot blot assay. Because thrombopoietin is a secreted protein, it is very possible that it may be washed away from your cells during the staining process. If you are not satisfied with these antibodies, I will be more than happy to offer you a refund or a credit. Please let me know how you would like to proceed and I will be happy to help you further.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2012

    Question

    I know that your company send me the details how to refund on off the antibodys. But I can’t do it via this way because it doesn’t fit with our computers here.

    Answer ur question:

    1) WB, high background, now Bands in the Western Blot

    IF, high background and none specific Binding

    FACS, only background but now speciffic binding

    2) Antibody dilutions

    ab93909 2µg/ml to 10µg/ml FACS, IF and WB

    ab128487 1: 50 bis 1:125 FACS, IF and WB

    3) 20µg of Protein been loaded tot he WB like all off my WB looks like

    4) I tried my old protocoll which I send u last time, we fixed the cells and +/-permeabilisation I tried too

    5) The second antiboys are perfect because i use them for other IF, WB and FACS too and there they are looking perfect

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    Thank you for your reply and the additional information.

    Regarding the refund, you would need to let your purchasing department know the information I had sent and they then should contact us so that the refund can be issued to you. I'm certain there is a way this can be arranged after your institution gets in touch with us.

    Regarding the other 2 antibodies:
    Your protocol in general looked fine and the antibody dilution used are more concentrated than the suggested range.

    You mentioned that you used Hela cells and I am wondering - since 3 anti-thrombopoietin antibodies seem not to be working - if this protein is expressed in Hela cells.

    It is mainly present in megakaryocytes and megakaryocytes-derived cells.
    http://www.proteinatlas.org/ENSG00000090534
    http://www.uniprot.org/uniprot/P40225

    The protein was shown to be present also in MCF7 cells, liver tissue, HepG2 cells.
    What other cell types have you tried, besides Hela cells? Would you be able to try any of the positive controls?

    I look forward to hear back from you and help you further.

    Read More

    Abcam Scientific Support

    Answered on Nov 07 2012

    Question

    the answer to your question:
    The problems are not solved.
    All 3 antibodys which I bought sre not function in our hands.
    Teh andtibodys are:
    Ab128487
    Ab93909
    Ab40664
    My collegues and I checked them all 3 in 3 different methods.
    Western Blotting, FACS and IF
    We've been using Hela cells
    Protocolls from the WB, IF and FACS i put in a word file for you.
    I am not happy about this situation but I can't change the situation.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 06 2012

    Answer

    Thank you for contacting us.
    I am sorry to hear that these antibodies do not work as expected.

    According to the records in our system, the purchase of ab40664 has already been refunded and the case has been discussed and resolved.

    As for ab93909 and ab128487 I have a few more questions, if you don't mind:

    1) What is the actual problem you have encountered (e.g. no bands in WB, high background in ICC/IF etc)?
    I just wanted to mention that ab93909 has not been tested in FACS and ab128487 has not been tested in ICC/IF, but a discount code was issued to you for trying the latter antibody in ICC/IF.

    2) What antibody dilutions were used?

    3) How much protein was loaded onto the gel for WB analysis?

    4) For flow cytometry, did you try fixing and permeabilizing the cells?

    5) How well are the secondary antibodies working with other primary antibodies?


    I look forward to hear back from you and assist you further.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.