Anti-Thrombopoietin antibody (ab40664)
Key features and details
- Rabbit polyclonal to Thrombopoietin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombopoietin antibody
See all Thrombopoietin primary antibodies -
Description
Rabbit polyclonal to Thrombopoietin -
Host species
Rabbit -
Specificity
Cross reactivity: Thrombopoietin Human (299-332) 100%; Thrombopoietin Human recombinant ~90% Does not cross react with Erythropoietin Human recombinant. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide: PPTGQYTLFP LPPTLPTPVV QLHPLLPDPS APTP conjugated to KLH, corresponding to amino acids 299-332 of Human Thrombopoietin Peptide available as ab93626.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 21149592
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 21149592 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
Target
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Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
Sequence similarities
Belongs to the EPO/TPO family. -
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7066 Human
- Omim: 600044 Human
- SwissProt: P40225 Human
- Unigene: 1166 Human
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Alternative names
- C mpl ligand antibody
- C-mpl ligand antibody
- Megakaryocyte colony stimulating factor antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab40664 has been referenced in 1 publication.
- Hoffmann O et al. Thrombopoietin contributes to neuronal damage in experimental bacterial meningitis. Infect Immun 79:928-36 (2011). IHC-P ; Human . PubMed: 21149592