Question (66245) | Anti-Thrombopoietin antibody (ab93909)

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Question

we transafected and transfecting every week cMPL ( Receptor of Thrombopietin ) and put rTHPO into the cell culture to find out the bindung way. But the Problem ist that all the antobodys which we tried from you are not working in this way!

Answer

Thank you for your reply. If I am understanding your assay correctly, your cells are overexpressing a thrombopoietin receptor and you are incubating the cell culture with recombinant thrombopoietin to measure the binding efficacy. What type of recombinant thrombopoietin are you using? It would be very helpful if you could let me know the manufacturer and the catalog number. Have you tried a dot blot or WB with the recombinant protein to see if it is recognized by these antibodies? If you are able to detect the recombinant protein by itsself, there may be an issue with the receptor. I hope this helps, please let me know if you need any additional information or assistance.

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