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my cells are overexpressing a thrombopoietin receptor and we are incubating the cell culture with recombinant thrombopoietin to measure different things.
Recombinant Human Thrombopoietin
I trieted my cells with TPO and i have done a lot of WB with other antibodies and they are all fine,.
But the antibodies which i bought from you do not function in WB; IF and FACS in my/ our hands.
Asked on Nov 09 2012
Thank you for your reply. I would still recommend checking if the antibodies bind to the recombinant protein in a dot blot assay. Because thrombopoietin is a secreted protein, it is very possible that it may be washed away from your cells during the staining process. If you are not satisfied with these antibodies, I will be more than happy to offer you a refund or a credit. Please let me know how you would like to proceed and I will be happy to help you further.
Answered on Nov 09 2012