Anti-Thrombospondin 1 antibody (ab226383)
Key features and details
- Rabbit polyclonal to Thrombospondin 1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Thrombospondin 1 antibody
See all Thrombospondin 1 primary antibodies -
Description
Rabbit polyclonal to Thrombospondin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Thrombospondin 1 aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P07996 -
Positive control
- WB: HeLa and TCMK-1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226383 was affinity purified using an epitope specific to Thrombospondin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 129 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 129 kDa. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
Cellular localization
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 7057 Human
- Entrez Gene: 21825 Mouse
- Entrez Gene: 492313 Pig
- Omim: 188060 Human
- SwissProt: P07996 Human
- SwissProt: P35441 Mouse
- SwissProt: Q8CGB2 Mouse
- Unigene: 164226 Human
see all -
Alternative names
- Thbs1 antibody
- Thrombospondin-1 antibody
- TSP antibody
see all
Images
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Western blot - Anti-Thrombospondin 1 antibody (ab226383)All lanes : Anti-Thrombospondin 1 antibody (ab226383) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDa
Exposure time: 3 minutesLysates prepared using RIPA lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab226383 has been referenced in 1 publication.
- Kan L et al. TMT-based quantitative proteomic analysis of antitumor mechanism of Sporisorium reilianum polysaccharide WM-NP-60 against HCT116 cells. Int J Biol Macromol 165:1755-1764 (2020). PubMed: 33068624
