Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Thrombospondin 2 antibody
See all Thrombospondin 2 primary antibodies
DescriptionRabbit polyclonal to Thrombospondin 2
SpecificityReactivity in Rat is no longer guaranteed due to difficulty reproducing in-house experimental evidence, but will remain predicted, due to the immunogen showing 100% xr with Rat.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog
Synthetic peptide corresponding to Human Thrombospondin 2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate. This antibody gave a positive signal in ICC/IF in U2O2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab84469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.
Tissue specificityHigh expression in invertebral disk tissue.
Involvement in diseaseGenetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
- Information by UniProt
- THBS2 antibody
- Thrombospondin-2 antibody
- TSP2 antibody
- TSP2_HUMAN antibody
Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).
All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml
Lane 1 : Mouse spinal cord embryo E14
Lane 2 : Human skin
Lane 3 : Mouse skin
Lane 4 : A431 (Human epithelial carcinoma cell line)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)
Exposure time: 20 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab84469 has been referenced in 6 publications.
- Pan W et al. TSP2 acts as a suppresser of cell invasion, migration and angiogenesis in medulloblastoma by inhibiting the Notch signaling pathway. Brain Res 1718:223-230 (2019). PubMed: 31063715
- Wu XG et al. Cancer-derived exosomal miR-221-3p promotes angiogenesis by targeting THBS2 in cervical squamous cell carcinoma. Angiogenesis N/A:N/A (2019). PubMed: 30993566
- Guo Y et al. Molecular characterization and functional analysis of Japanese flounder (Paralichthys olivaceus) thbs2 in response to lymphocystis disease virus. Fish Shellfish Immunol 93:183-190 (2019). PubMed: 31330254
- Du P et al. Downregulation of microRNA-1246 inhibits tumor growth and promotes apoptosis of cervical cancer cells by targeting thrombospondin-2. Oncol Lett 18:2491-2499 (2019). PubMed: 31404330
- Shen Y et al. MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model. Acta Cir Bras 34:e201900602 (2019). PubMed: 31432993
- Wang S et al. Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure. PLoS One 12:e0185388 (2017). PubMed: 28953973