Anti-Thrombospondin antibody [A6.1] (ab1823)
- Datasheet
- References (33)
- Protocols
Overview
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Product nameAnti-Thrombospondin antibody [A6.1]
See all Thrombospondin primary antibodies -
DescriptionMouse monoclonal [A6.1] to Thrombospondin
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
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Immunogen
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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Positive control
- Tonsil
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberA6.1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab1823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
Target
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FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
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Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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Database links
- Entrez Gene: 281530 Cow
- Entrez Gene: 487486 Dog
- Entrez Gene: 100057478 Horse
- Entrez Gene: 7057 Human
- Entrez Gene: 21825 Mouse
- Entrez Gene: 492313 Pig
- Entrez Gene: 445442 Rat
- Entrez Gene: 100294615 Sheep
see all -
Alternative names
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/ab1823-275744-ab1823-Supplier-IHC5.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.
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![Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-39.jpg)
Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreviewab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/ab1823_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
Protocols
References
This product has been referenced in:
- Middleton RC et al. Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes. J Extracell Vesicles 7:1456888 (2018). Read more (PubMed: 29696078) »
- Hiratsuka S et al. Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29930175) »
