Anti-Thrombospondin antibody [A6.1] (ab1823)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/ab1823-275744-ab1823-Supplier-IHC5.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)")
Key features and details
- Mouse monoclonal [A6.1] to Thrombospondin
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Thrombospondin antibody [A6.1]
See all Thrombospondin primary antibodies -
Description
Mouse monoclonal [A6.1] to Thrombospondin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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Positive control
- Tonsil
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
A6.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| IHC-P | (7) |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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Database links
- Entrez Gene: 7057 Human
- Entrez Gene: 445442 Rat
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
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Alternative names
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)
Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.
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![Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-39.jpg)
Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreviewab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.com/ps/products/1/ab1823/Images/ab1823_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab1823 has been referenced in 56 publications.
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Semba S et al. Synthetic PAMPS gel induces chondrogenic differentiation of ATDC5 cells via a novel protein reservoir function. J Biomed Mater Res A N/A:N/A (2020). PubMed: 32496623
- Wang TY et al. Maggot excretions/secretions promote diabetic wound angiogenesis via miR18a/19a - TSP-1 axis. Diabetes Res Clin Pract 165:108140 (2020). PubMed: 32277954
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
- Shih HJ et al. IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res 10:1728-1744 (2020). PubMed: 32642286