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    thrombospondin-antibody-a61-ab1823.pdf

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Neuroscience Cell Type Marker Glia marker Astrocyte marker
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Anti-Thrombospondin antibody [A6.1] (ab1823)

  • Datasheet
  • SDS
Reviews (19)Q&A (25)References (56)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)
  • Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)

Key features and details

  • Mouse monoclonal [A6.1] to Thrombospondin
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Thrombospondin antibody [A6.1]
    See all Thrombospondin primary antibodies
  • Description

    Mouse monoclonal [A6.1] to Thrombospondin
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

  • Positive control

    • Tonsil
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    A6.1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Platelets
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Regulation
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Secreted
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Thrombospondin protein (ab52124)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (4)
Use at an assay dependent concentration.
IHC-P (7)
Use at an assay dependent concentration.
Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  • Sequence similarities

    Belongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Target information above from: UniProt accession P07996 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7057 Human
    • Entrez Gene: 445442 Rat
    • Omim: 188060 Human
    • SwissProt: P07996 Human
    • Unigene: 164226 Human
    • Alternative names

      • THBS 1 antibody
      • THBS antibody
      • Thbs1 antibody
      • Thrombospondin 1 antibody
      • Thrombospondin-1 antibody
      • TSP 1 antibody
      • TSP antibody
      • TSP1 antibody
      • TSP1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)

      Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.

    • Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreview
      ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)
      ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)

      ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .

      This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (56)

    Publishing research using ab1823? Please let us know so that we can cite the reference in this datasheet.

    ab1823 has been referenced in 56 publications.

    • Qu S  et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
    • Semba S  et al. Synthetic PAMPS gel induces chondrogenic differentiation of ATDC5 cells via a novel protein reservoir function. J Biomed Mater Res A N/A:N/A (2020). PubMed: 32496623
    • Wang TY  et al. Maggot excretions/secretions promote diabetic wound angiogenesis via miR18a/19a - TSP-1 axis. Diabetes Res Clin Pract 165:108140 (2020). PubMed: 32277954
    • Fu Y  et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
    • Shih HJ  et al. IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res 10:1728-1744 (2020). PubMed: 32642286
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-10 of 19 Abreviews

    Western blot abreview for Anti-Thrombospondin antibody [A6.1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Serum (Rabbit Aqueouse Humor, 20ul/well)
    Gel Running Conditions
    Reduced Denaturing (8-20%)
    Loading amount
    20 µg
    Specification
    Rabbit Aqueouse Humor, 20ul/well
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Xiaoqing Guo

    Verified customer

    Submitted Apr 30 2019

    Western blot abreview for Anti-Thrombospondin antibody [A6.1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% mini gel)
    Sample
    Mouse Tissue lysate - whole (Penile Tissue)
    Specification
    Penile Tissue
    Blocking step
    SuperBlock Blocking Buffer (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Miss. Gwen Lagoda

    Verified customer

    Submitted Nov 11 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Pig Tissue sections (liver)
    Specification
    liver
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 29 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Rabbit Tissue sections (splen rabbit)
    Specification
    splen rabbit
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEFs)
    Specification
    MEFs
    Fixative
    Formaldehyde
    Permeabilization
    Yes - TBS Tw20
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 12 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (1BRG3 fibroblast)
    Specification
    1BRG3 fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Tween 20
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 12 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Meriones unguiculatus Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Ms. Andrea Dannullis

    Verified customer

    Submitted Sep 25 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (intestinum)
    Specification
    intestinum
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    Yes - PBS/TritonX
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsille)
    Specification
    tonsille
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2012

    1-10 of 19 Abreviews

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