Key features and details
- Goat polyclonal to Thrombospondin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thrombospondin antibody
See all Thrombospondin primary antibodies
DescriptionGoat polyclonal to Thrombospondin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HEK-293 cells overexpressing human thrombospondin with C-terminal MYC tag.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223580 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 129 kDa).
1 hour primary incubation is recommended for this product.
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
- Information by UniProt
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
Lanes 1-2 : Anti-Thrombospondin antibody (ab223580) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)
Lane 2 : Mock-transfected HEK-293 cells lysate (in RIPA buffer)
Lane 3 : HEK-293 cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubations were for 1 hour.
ab223580 has not yet been referenced specifically in any publications.