Overview

  • Product name

    Anti-Thrombospondin antibody
    See all Thrombospondin primary antibodies
  • Description

    Goat polyclonal to Thrombospondin
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Thrombospondin aa 475-487 (internal sequence). (NP_003237.2).
    Sequence:

    EGEARETKACKKD


    Database link: P079962

  • Positive control

    • WB: HEK-293 cells overexpressing human thrombospondin with C-terminal MYC tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab223580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 129 kDa).

Target

  • Function

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  • Sequence similarities

    Belongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • THBS 1 antibody
    • THBS antibody
    • Thbs1 antibody
    • Thrombospondin 1 antibody
    • Thrombospondin-1 antibody
    • TSP 1 antibody
    • TSP antibody
    • TSP1 antibody
    • TSP1_HUMAN antibody
    see all

Images

  • Lanes 1-2 : Anti-Thrombospondin antibody (ab223580) at 1 µg/ml
    Lane 3 : Anti-Myc tag at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)
    Lane 2 : Mock-transfected HEK-293 cells lysate (in RIPA buffer)
    Lane 3 : HEK-293 cell lysate overexpressing human Thrombospondin with C-terminal Myc tag (in RIPA buffer)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 129 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



     Primary incubations were for 1 hour.

References

ab223580 has not yet been referenced specifically in any publications.

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