Overview

  • Product name

    Anti-Thrombospondin antibody
    See all Thrombospondin primary antibodies
  • Description

    Rabbit polyclonal to Thrombospondin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Thrombospondin aa 300-350. The exact sequence is proprietary.
    Database link: P07996

  • Positive control

    • WB: HeLa and TCMK-1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 129 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-Thrombospondin antibody (ab226383) at 0.4 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 129 kDa


      Exposure time: 3 minutes


      Lysates prepared using RIPA lysis buffer.

    References

    ab226383 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rabbit Serum (aqueous humor from rabbit eyes)
    Gel Running Conditions
    Reduced Denaturing (8-20%)
    Loading amount
    20 µg
    Specification
    aqueous humor from rabbit eyes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Xiaoqing Guo

    Verified customer

    Submitted Jun 25 2019

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