Key features and details
- Mouse polyclonal to Thrombospondin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thrombospondin antibody
See all Thrombospondin primary antibodies
DescriptionMouse polyclonal to Thrombospondin
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human Thrombospondin protein (NP_003237.2)
- WB: Human pancreas tissue lysate and Thrombospondin transfected 293T cell lysate IF: HeLa cell
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
- Information by UniProt
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
All lanes : Anti-Thrombospondin antibody (ab88529) at 1 µg/ml
Lane 1 : Thrombospondin transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 129 kDa
ab88529 at 10 µg/ml staining Thrombospondin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
Anti-Thrombospondin antibody (ab88529) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 129 kDa
Observed band size: ~ 150 kDa why is the actual band size different from the predicted?
ab88529 has been referenced in 5 publications.
- Duan S et al. Effects of taxol resistance gene 1 on the cisplatin response in gastric cancer. Oncol Lett 15:8287-8294 (2018). PubMed: 29805561
- Liu Y et al. MicroRNA-182-5p contributes to the protective effects of thrombospondin 1 against lipotoxicity in INS-1 cells. Exp Ther Med 16:5272-5279 (2018). PubMed: 30546417
- Patel GK et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). PubMed: 28152544
- Xu C et al. Circadian Clock Regulates Bone Resorption in Mice. J Bone Miner Res 31:1344-55 (2016). PubMed: 26841172
- Bonazzi VF et al. Cross-Platform Array Screening Identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as Genes Frequently Silenced by Methylation in Melanoma. PLoS One 6:e26121 (2011). WB ; Human . PubMed: 22028813