Key features and details
- Goat polyclonal to Thrombospondin - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thrombospondin antibody - N-terminal
See all Thrombospondin primary antibodies
DescriptionGoat polyclonal to Thrombospondin - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- WB: HEK-293 lysate overexpressing Human Thrombospondin with C-terminal MYC tag.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223472 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 129 kDa.
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
- Information by UniProt
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
Anti-Thrombospondin antibody - N-terminal (ab223472) at 1 µg/ml + Mouse kidney lysate at 35 µg
Predicted band size: 129 kDa
Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ab223472. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
Ab223472 (5µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Lanes 1-2 : Anti-Thrombospondin antibody - N-terminal (ab223472) at 0.1 µg/ml
Lane 3 : anti-MYC Tag at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) lysate overexpressing Human Thrombospondin with C-terminal MYC (in RIPA buffer)
Lane 2 : Mock transfected HEK-293 lysate (in RIPA buffer)
Lane 3 : HEK-293 lysate overexpressing Human Thrombospondin with C-terminal MYC (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDa
Primary incubations were for 1 hour.
In transfected HEK-293 transiently expressing Human Thrombospondin a band of approx. 200kDa plus a breakdown product are observed. These bands are not observed in the non-transfected HEK-293.
ab223472 has not yet been referenced specifically in any publications.