Key features and details
- Rabbit polyclonal to Thrombospondin - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thrombospondin antibody - N-terminal
See all Thrombospondin primary antibodies
DescriptionRabbit polyclonal to Thrombospondin - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment within Human Thrombospondin (N terminal). The exact sequence is proprietary.
Database link: P07996
- ICC/IF: HUVEC cells. WB: HUVEC whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226950 in the following tested applications.
|WB||1/1000 - 1/10000. Predicted molecular weight: 129 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
- Information by UniProt
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
HUVEC (human umbilical vein endothelial cell line) cells stained for Thrombospondin (green) using ab226950 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
Blue: Hoechst 33342 staining.
Anti-Thrombospondin antibody - N-terminal (ab226950) at 1/4000 dilution + HUVEC (Human umbilical vein endothelial cell line) whole cell extract at 30 µg
Predicted band size: 129 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226950 has not yet been referenced specifically in any publications.