Product nameThrombospondin overexpression 293T lysate (whole cell)
ab94095 is a 293T cell transfected lysate in which Human Thrombospondin has been transiently over-expressed using a pCMV-Thrombospondin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Similarity: Belongs to the thrombospondin family. Contains 3 EGF-like domains. Contains 1 TSP C-terminal (TSPC) domain. Contains 1 TSP N-terminal (TSPN) domain. Contains 3 TSP type-1 domains. Contains 8 TSP type-3 repeats. Contains 1 VWFC domain.
Our Abpromise guarantee covers the use of ab94095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94095 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Thrombospondin antibody (ab88529) at 1/500 dilution
Lane 1 : Thrombospondin 293T Transfected Lysate - Positive Control (ab94095)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94095 has not yet been referenced specifically in any publications.