Overview

  • Product name

    Thromboxane B2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 24 759pg/ml = 3.6%
    Medium 24 1283pg/ml = 4%
    High 24 2605pg/ml = 1.6%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 44pg/ml = 7.6%
    Medium 8 371pg/ml = 3.6%
    High 8 3053pg/ml = 6.2%
  • Sample type

    Saliva, Urine, Serum, Tissue Culture Media, Heparin Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 10.54 pg/ml
  • Range

    13.7 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva = 98.3 % - %
    Urine = 102.2 % - %
    Serum = 113 % - %
    Tissue Culture Media = 104.7 % - %
    Heparin Plasma = 111 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Thromboxane B2 (TXB2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B2 in biological fluids.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB2 antigen and a rabbit polyclonal antibody specific to TXB2. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB2 captured in the plate.

  • Notes

    Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.

    Cross Reactivity 

    Compound  Cross Reactivity
     TXB2 100%
     2,3-dinorTXB2 7.1%
     11-dehydro TXB2 0.4%
     6-keto-PGF 0.23%
     PGD2 <0.1%
     PGE2<0.1%
     PGF <0.1%
     PGF  <0.1%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 27ml
    Assay Buffer 1 x 27ml
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Plate Sealer 1 unit
    pNpp Substrate 1 x 20ml
    Rabbit Polyclonal Thromboxane B2 Antibody 1 x 5ml
    Stop Solution 1 x 5ml
    Thromboxane B2 Alkaline Phosphatase Conjugate 1 x 5ml
    Thromboxane B2 Standard 1 x 500µl
  • Research areas

  • Alternative names

    • T box 2
    • T box transcription factor TBX2
    • T-box protein 2
    • T-box transcription factor TBX2
    • Thromboxane B2
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab133022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  •  Representative Standard Curve using ab133022.

Protocols

References

ab133022 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer



We sell indomethacin as catalog number ab120719.
https://www.abcam.com/indomethacin-ab120719.html

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Answer

The inhibitors should be added as soon as possible after collection. If samples were frozen already then it would be wise to added them as soon as the sample thaws. The inhibitors are there to prevent additional production of prostaglandins post collection so the sooner you add the inhibitors the better. These inhibitors should be added to all sample types for the most accurate results.

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