Product nameAnti-Thromboxane synthase antibody
See all Thromboxane synthase primary antibodies
DescriptionRabbit polyclonal to Thromboxane synthase
Tested applicationsSuitable for: WB, IHC-Fr, IHC-FoFr, ICC, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cynomolgus monkey
- Whole cell lysate from platelets.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab39362 is an affinity purified IgG
Our Abpromise guarantee covers the use of ab39362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Tissue specificityPlatelets, lung, kidney, spleen, macrophages and lung fibroblasts.
Involvement in diseaseDefects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD) [MIM:231095]. GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all.
Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency) [MIM:274180]. It is characterized by hemorrhagic diathesis.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- BDPLT14 antibody
- CYP5 antibody
- CYP5A1 antibody
All lanes : Anti-Thromboxane synthase antibody (ab39362) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This product has been referenced in:
- Pai CH et al. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy. PLoS One 11:e0151617 (2016). IHC . Read more (PubMed: 26974824) »
- Font-Nieves M et al. Induction of COX-2 Enzyme and Down-regulation of COX-1 Expression by Lipopolysaccharide (LPS) Control Prostaglandin E2 Production in Astrocytes. J Biol Chem 287:6454-68 (2012). WB ; Mouse . Read more (PubMed: 22219191) »