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All samples are from mouse tissues (visceral fat). The samples were homogenized with the cell lysis buffer (Cell Signaling) including protease inhibitors.
Yes, the samples were reduced and denatured before loading into the gel.
We also ordered the anti-FADS1 antibody (ab 126706) and used it on our blot. We got the right predicted band with it.
Asked on Nov 08 2012
Thank you for the additional information about your samples and protocol. I will be happy to issue a refund if you like. If you are interested, we also have another antibody which I recommend as a free replacement, ab68823:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=68823).
Although it has not been tested on mouse samples, reactivity is likely, given that the immunogen sequence is 100% conserved in mouse TBXAS1. Please let me know how you would like to proceed.
Answered on Nov 08 2012