Key features and details
- Mouse monoclonal [TARSF8H3] to ThrRS
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-ThrRS antibody [TARSF8H3]
See all ThrRS primary antibodies
DescriptionMouse monoclonal [TARSF8H3] to ThrRS
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ThrRS.
- WB: HeLa whole cell lysate (ab150035). ICC/IF: HeLa cell.
Product previously known as Mouse monoclonal [TARSF8H3] to TARS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. It is sterile filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab50795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).|
Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
- Information by UniProt
- cytoplasmic antibody
- MGC9344 antibody
- SYTC_HUMAN antibody
Anti-ThrRS antibody [TARSF8H3] (ab50795) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Ab50795, at a dilution of 1/5, staining ThrRS in HeLa cell by Immunocytochemistry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
ab50795 has not yet been referenced specifically in any publications.