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Anti-THUMPD1 antibody (ab199850)

  • Datasheet
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Western blot - Anti-THUMPD1 antibody (ab199850)
  • Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)

Key features and details

  • Rabbit polyclonal to THUMPD1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-THUMPD1 antibody
  • Description

    Rabbit polyclonal to THUMPD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human THUMPD1 aa 303-353 (C terminal). The exact sequence is proprietary. (NP_060206.2).
    Sequence:

    GKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDF S


    Database link: Q9NXG2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T and Jurkat cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab199850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 39 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the THUMPD1 family.
    Contains 1 THUMP domain.
  • Target information above from: UniProt accession Q9NXG2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55623 Human
    • SwissProt: Q9NXG2 Human
    • Unigene: 741402 Human
    • Alternative names

      • DKFZp686C1054 antibody
      • FLJ20274 antibody
      • THUM1_HUMAN antibody
      • THUMP domain containing 1 antibody
      • THUMP domain containing protein 1 antibody
      • THUMP domain-containing protein 1 antibody
      • THUMPD 1 antibody
      • THUMPD1 antibody
      see all

    Images

    • Western blot - Anti-THUMPD1 antibody (ab199850)
      Western blot - Anti-THUMPD1 antibody (ab199850)
      All lanes : Anti-THUMPD1 antibody (ab199850) at 0.1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 39 kDa


      Exposure time: 10 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)
      Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)

      Detection of THUMPD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199850 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

       

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab199850? Please let us know so that we can cite the reference in this datasheet.

    ab199850 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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