Anti-THUMPD1 antibody (ab199850)

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Overview

  • Product name

    Anti-THUMPD1 antibody

  • Description

    Rabbit polyclonal to THUMPD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee

  • Immunogen

    Synthetic peptide within Human THUMPD1 aa 303-353 (C terminal). The exact sequence is proprietary. (NP_060206.2).
    Sequence:

    GKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDF S


    Database link: Q9NXG2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, 293T and Jurkat cell lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 39 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the THUMPD1 family.
    Contains 1 THUMP domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686C1054 antibody
      • FLJ20274 antibody
      • THUM1_HUMAN antibody
      • THUMP domain containing 1 antibody
      • THUMP domain containing protein 1 antibody
      • THUMP domain-containing protein 1 antibody
      • THUMPD 1 antibody
      • THUMPD1 antibody
      see all

    Images

    • Western blot - Anti-THUMPD1 antibody (ab199850)
      Western blot - Anti-THUMPD1 antibody (ab199850)
      All lanes : Anti-THUMPD1 antibody (ab199850) at 0.1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 39 kDa


      Exposure time: 10 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)
      Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)

      Detection of THUMPD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199850 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

       

    References

    ab199850 has not yet been referenced specifically in any publications.

    Publishing research using ab199850? Please let us know so that we can cite the reference in this datasheet.

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