The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.03-0.06µg for 105-8 cells.
A final volume of 100 µL is recommended for staining the cell sample.
ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.