Validated using a knockout cell line

Anti-Thymidine Kinase 1 antibody [EPR3194] (ab91651)


  • Product name
    Anti-Thymidine Kinase 1 antibody [EPR3194]
    See all Thymidine Kinase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3194] to Thymidine Kinase 1
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Hela cells and Molt-4 cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab91651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Sequence similarities
      Belongs to the thymidine kinase family.
    • Post-translational
      Phosphorylated on Ser-13 in mitosis.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • cytosolic antibody
      • KITH_HUMAN antibody
      • Thymidine kinase 1 antibody
      • Thymidine kinase 1 soluble antibody
      • Thymidine kinase 1 soluble isoform antibody
      • Thymidine kinase antibody
      • Thymidine kinase cytosolic antibody
      • TK 1 antibody
      • TK 2 antibody
      • TK1 antibody
      • Tk1a antibody
      • Tk1b antibody
      • TK2 antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  Ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • ab91651 at 1/100 dilution staining Thymidine kinase 1 in Hela cells
    • Anti-Thymidine Kinase 1 antibody [EPR3194] (ab91651) at 1/500 dilution + Molt-4 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 25 kDa


    This product has been referenced in:
    • Weagel EG  et al. Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemia. Onco Targets Ther 10:4355-4367 (2017). Read more (PubMed: 28919785) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry.

    I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

    Read More

    Thank you for contacting Abcam.

    The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

    Also for ab76495, we guarantee it to react with mouse and human tissue in the following appl...

    Read More

    Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-termin...

    Read More


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