Product nameThymidine Kinase 1 overexpression 293T lysate (whole cell)
DescriptionThymidine Kinase 1 293T Transfected Lysate - (positive control)
ab94157 is a 293T cell transfected lysate in which Human Thymidine Kinase 1 has been transiently over-expressed using a pCMV-Thymidine Kinase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundSimilarity: Belongs to the thymidine kinase family. PTM: Phosphorylated on Ser-13 in mitosis.
Our Abpromise guarantee covers the use of ab94157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94157 at 15 µg/lane on an SDS-PAGE gel
All lanes : Anti-Thymidine Kinase 1/TK1 antibody (ab57757) at 1/500 dilution
Lane 1 :
Thymidine Kinase 1 overexpression 293T lysate (whole cell) (ab94157)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94157 has not yet been referenced specifically in any publications.