Key features and details
- Rabbit polyclonal to Thymidine Kinase 1/TK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thymidine Kinase 1/TK1 antibody
See all Thymidine Kinase 1/TK1 primary antibodies
DescriptionRabbit polyclonal to Thymidine Kinase 1/TK1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human Thymidine Kinase 1/TK1.
Database link: NP_003249.1
- HeLa and 293T whole cell lysates.
This product was previously labelled as Thymidine Kinase 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
Our Abpromise guarantee covers the use of ab75277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
Sequence similaritiesBelongs to the thymidine kinase family.
modificationsPhosphorylated on Ser-13 in mitosis.
- Information by UniProt
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
All lanes : Anti-Thymidine Kinase 1/TK1 antibody (ab75277) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 130 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab75277 has not yet been referenced specifically in any publications.