Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Thymidine Kinase 1/TK1 antibody [EPR3193] - BSA and Azide free (ab239885)

Overview

  • Product name

    Anti-Thymidine Kinase 1/TK1 antibody [EPR3193] - BSA and Azide free
    See all Thymidine Kinase 1/TK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3193] to Thymidine Kinase 1/TK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thymidine Kinase 1/TK1 aa 1-100. The exact sequence is proprietary.

  • General notes

    ab239885 is the carrier-free version of ab76495 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239885 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.  This product was previously labelled as Thymidine Kinase 1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3193
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab239885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Sequence similarities

    Belongs to the thymidine kinase family.
  • Post-translational
    modifications

    Phosphorylated on Ser-13 in mitosis.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 ab76495

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76495).

  • Overlay histogram showing HeLa cells stained with ab76495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76495, 1/50 dilution) for 30 min at 22�C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22�C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76495).

References

ab239885 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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