Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)

Submit a review Q&A (3)References (3)
Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
  • Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
  • Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Thymidine Kinase 1/TK1 antibody [EPR3194]
    See all Thymidine Kinase 1/TK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Thymidine Kinase 1/TK1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. Jurkat and MOLT-4 whole cell lysate. ICC/IF: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Thymidine Kinase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3194

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Sequence similarities

      Belongs to the thymidine kinase family.

    • Post-translational
      modifications

      Phosphorylated on Ser-13 in mitosis.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • cytosolic antibody
        • KITH_HUMAN antibody
        • Thymidine kinase 1 antibody
        • Thymidine kinase 1 soluble antibody
        • Thymidine kinase 1 soluble isoform antibody
        • Thymidine kinase antibody
        • Thymidine kinase cytosolic antibody
        • TK 1 antibody
        • TK 2 antibody
        • TK1 antibody
        • Tk1a antibody
        • Tk1b antibody
        • TK2 antibody
        see all

      Images

      • Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
        Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)

        Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
        Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
        Lane 3: Jurkat whole cell lysate (20 µg)
        Lane 4: MOLT4 whole cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

      • Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
        Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)

        ab91651 at 1/100 dilution staining Thymidine kinase 1/TK1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      • Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
        Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
        Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651) at 1/500 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

        Secondary
        HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 25 kDa

      • Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)
        Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651)

      References (3)

      Publishing research using ab91651? Please let us know so that we can cite the reference in this datasheet.

      ab91651 has been referenced in 3 publications.

      • Velazquez EJ  et al. Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells. Cancer Cell Int 20:127 (2020). PubMed: 32317865
      • Weagel EG  et al. Membrane expression of thymidine kinase 1 and potential clinical relevance in lung, breast, and colorectal malignancies. Cancer Cell Int 18:135 (2018). PubMed: 30214377
      • Weagel EG  et al. Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemia. Onco Targets Ther 10:4355-4367 (2017). PubMed: 28919785

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Product code: 91651

      Inquiry: To whom it may concern, I am looking for an antibody againt TK-1 for paraffin embedded mouse tumour tissue and was wondering whether you had any recommendations on positive and negative control tissue. I am specifically asking as the only information about positive control tissue I found is the human tonsil which is not applicable in mice . Many thanks for your help! Regards, Kathrin

      Read More
      Answer

      Thank you for your enquiry.

      I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated as soon as we have any new information.

      The antibody has also been tested unsuccessfully in mouse, as stated on the datasheet. Therefore, regrettably this is not the most suitable antibody for your experiments.

      I can suggest one of the following two, tested in IHC-P and mouse, would be more suitable and will be covered by our 6 month guarantee in this application and species. I can recommend to review the online datasheets for further details:

      ab59271 Anti-Thymidine Kinase 1 antibody
      Tested in: IHC-P, WB, ELISA
      Rabbit polyclonal
      Reacts with:Human, Mouse
      https://www.abcam.com/Thymidine-Kinase-1TK1-antibody-ab59271.html (or use the following: https://www.abcam.com/Thymidine-Kinase-1TK1-antibody-ab59271.html).

      ab76495 2563-1 Anti-Thymidine Kinase 1 antibody [EPR3193]
      Tested in: Flow Cyt, IP, WB, ICC/IF, IHC-P
      Rabbit monoclonal
      Reacts with: Human, Mouse
      https://www.abcam.com/Thymidine-Kinase-1TK1-antibody-EPR3193-ab76495.html (or use the following: https://www.abcam.com/Thymidine-Kinase-1TK1-antibody-EPR3193-ab76495.html).

      According to the EST expression profile on the Unigen database link on the datasheet, in mouse the Tk1 protein is expressed quite highly in lymph node and several other tissues.
      http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.2661

      I can therefore recommend to consider lymph node as a positive control tissue. You may wish to do some further literature searching for more information.

      Read More

      Question

      I will be grateful if you could please answer my question regarding the 2x Anti-Thymidine Kinase 1 antibody which you show on your website.
      In particular, can you please let me know what is the difference between the rabbit monoclonal Anti-Thymidine Kinase [EPR3193] (ab76495), and the rabbit monoclonal Anti-Thymidine Kinase [EPR3194] (ab91651) one.
      I wish to use the Ab for immunohistochemistry on mouse tumor slices, but am unsure what the difference between the above 2 are.
      Thank you for attending to this email. I look forward to hearing from you.

      Read More
      Answer

      Thank you for contacting Abcam.

      The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

      Also for ab76495, we guarantee it to react with mouse and human tissue in the following applications, ICC/IF, WB, IP, IHC-P, Flow Cyt. Whereas ab91651 will react with human tissue but not mouse and rat and will work in the following applications WB, Flow Cyt, ICC/IF. Therefore ab91651 would not work for you since you wish to use the antibody on mouse sections.

      If there is anything else I can help you with, or if you need further selecting the most appropriate antibody for your experiments, please let me know.

      Read More

      Question

      Dear Tech Support Team, Kindly advise which of the following will specificaly recognize Thymidine Kinase 1 and will not cross react with Thymidine Kinase 2. ab76495 ab57757 ab91651

      Read More
      Answer

      Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-terminus (amino acids a-b) of human TK1 (SwissProt P04183), whereas ab91651 has its immunogen located at the C-terminus (amino acids y-z). These sequences do not share any homology with TK2. The TK1 antibody ab57757 has not been tested for a potential cross-reactivity with TK2. However, the immunogen is the full-length human TK1 which reveals no significant sequence similarity with human TK2 (SwissProt O00142). We therefore would not expect this antibody to cross-react with TK2. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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