Product nameAnti-Thymidine Kinase 1/TK1 antibody [EPR3194]
See all Thymidine Kinase 1/TK1 primary antibodies
DescriptionRabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Thymidine Kinase 1/TK1 aa 200-300. The exact sequence is proprietary.
- WB: Wild-type HAP1 whole cell lysate. Jurkat and MOLT-4 whole cell lysate. ICC/IF: HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Thymidine Kinase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab91651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 25 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
Sequence similaritiesBelongs to the thymidine kinase family.
modificationsPhosphorylated on Ser-13 in mitosis.
- Information by UniProt
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: MOLT4 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE. ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
ab91651 at 1/100 dilution staining Thymidine kinase 1/TK1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (ab91651) at 1/500 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
This product has been referenced in:
- Weagel EG et al. Membrane expression of thymidine kinase 1 and potential clinical relevance in lung, breast, and colorectal malignancies. Cancer Cell Int 18:135 (2018). Read more (PubMed: 30214377) »
- Weagel EG et al. Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemia. Onco Targets Ther 10:4355-4367 (2017). Read more (PubMed: 28919785) »