Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)

Overview

  • Product name

    Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free
    See all Thymidine Kinase 1/TK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thymidine Kinase 1/TK1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. Jurkat and MOLT-4 whole cell lysate. ICC/IF: HeLa cells.
  • General notes

    ab223164 is the carrier-free version of ab91651 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab223164 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3194
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Sequence similarities

      Belongs to the thymidine kinase family.
    • Post-translational
      modifications

      Phosphorylated on Ser-13 in mitosis.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • cytosolic antibody
      • KITH_HUMAN antibody
      • Thymidine kinase 1 antibody
      • Thymidine kinase 1 soluble antibody
      • Thymidine kinase 1 soluble isoform antibody
      • Thymidine kinase antibody
      • Thymidine kinase cytosolic antibody
      • TK 1 antibody
      • TK 2 antibody
      • TK1 antibody
      • Tk1a antibody
      • Tk1b antibody
      • TK2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE. ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91651).

    • ab91651 at 1/100 dilution staining Thymidine kinase 1/TK1 in Hela cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91651).

    References

    ab223164 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab223164.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up