Product nameAnti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free
See all Thymidine Kinase 1/TK1 primary antibodies
DescriptionRabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Thymidine Kinase 1/TK1 aa 200-300. The exact sequence is proprietary.
- WB: Wild-type HAP1 whole cell lysate. Jurkat and MOLT-4 whole cell lysate. ICC/IF: HeLa cells.
ab223164 is the carrier-free version of ab91651 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab223164 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab223164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Sequence similaritiesBelongs to the thymidine kinase family.
modificationsPhosphorylated on Ser-13 in mitosis.
- Information by UniProt
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: MOLT4 whole cell lysate (20 µg)
ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE. ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91651).
ab223164 has not yet been referenced specifically in any publications.