Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 (PE)
- Suitable for: ICC/IF, Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-Thymidine Kinase 1/TK1 antibody [EPR3194] (PE)
See all Thymidine Kinase 1/TK1 primary antibodies
DescriptionRabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Predicted to work with: Human
within Human Thymidine Kinase 1/TK1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P04183
- Flow Cyt: HeLa cells ICC/IF: HeLa cells
This product was previously labelled as Thymidine Kinase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab208998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the thymidine kinase family.
modificationsPhosphorylated on Ser-13 in mitosis.
- Information by UniProt
- cytosolic antibody
- KITH_HUMAN antibody
- Thymidine kinase 1 antibody
Overlay histogram showing HeLa cells stained with ab208998 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208998, 1/5000 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
ab208998 staining Thymidine Kinase 1/TK1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208998 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab208998 has not yet been referenced specifically in any publications.