Recombinant
RabMAb

Recombinant Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (Phycoerythrin) (ab208998)

Overview

  • Product name

    Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] (Phycoerythrin)
    See all Thymidine Kinase 1/TK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity


    Predicted to work with: Human
  • Immunogen

    within Human Thymidine Kinase 1/TK1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P04183

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as Thymidine Kinase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3194
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab208998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Flow Cyt 1/5000.

ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.

Target

  • Sequence similarities

    Belongs to the thymidine kinase family.
  • Post-translational
    modifications

    Phosphorylated on Ser-13 in mitosis.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab2208998 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208998, 1/5000 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • ab208998 staining Thymidine Kinase 1/TK1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208998 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab208998 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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