Key features and details
- Rabbit polyclonal to Thymidine Phosphorylase
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Thymidine Phosphorylase antibody
See all Thymidine Phosphorylase primary antibodies
DescriptionRabbit polyclonal to Thymidine Phosphorylase
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Thymidine Phosphorylase (internal sequence). The exact sequence is proprietary.
Database link: P19971
- WB: NCI-H929 whole cell extract. IHC-P: Human oral CA SCC25 xenograft tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
PathwayPyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2.
Involvement in diseaseMitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
- Information by UniProt
- ECGF 1 antibody
- ECGF antibody
- ECGF1 antibody
Paraffin-embedded human oral CA SCC25 xenograft tissue stained for Thymidine Phosphorylase using ab226917 at 1/100 dilution in immunohistochemical analysis.
Anti-Thymidine Phosphorylase antibody (ab226917) at 1/1000 dilution + NCI-H929 (human bone marrow B lymphoblast) whole cell extract at 30 µg
Predicted band size: 50 kDa
10% SDS-PAGE gel.
Methanol-fixed A431 (human epidermoid carcinoma cell line) cells labeling Thymidine Phosphorylase (green) using ab226917 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226917 has not yet been referenced specifically in any publications.