Anti-Thymidine Phosphorylase antibody - N-terminal (ab180783)
Key features and details
- Rabbit polyclonal to Thymidine Phosphorylase - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thymidine Phosphorylase antibody - N-terminal
See all Thymidine Phosphorylase primary antibodies -
Description
Rabbit polyclonal to Thymidine Phosphorylase - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Thymidine Phosphorylase aa 1-482 (N terminal).
Database link: P19971 -
Positive control
- A549 and THP1 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/50 - 1/200.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. -
Pathway
Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. -
Involvement in disease
Mitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. - Information by UniProt
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Database links
- Entrez Gene: 1890 Human
- Entrez Gene: 72962 Mouse
- Entrez Gene: 315219 Rat
- Omim: 131222 Human
- SwissProt: P19971 Human
- SwissProt: Q99N42 Mouse
- SwissProt: Q5FVR2 Rat
- Unigene: 180903 Human
see all -
Alternative names
- ECGF 1 antibody
- ECGF antibody
- ECGF1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Thymidine Phosphorylase with ab180783 at 1/100. Magnification: 200x.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180783. Blue DAPI for nuclear staining.
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All lanes : Anti-Thymidine Phosphorylase antibody - N-terminal (ab180783) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : THP1 cell lysate
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab180783 has been referenced in 2 publications.
- Wang Q et al. A predictive signature for oxaliplatin and 5-fluorouracil based chemotherapy in locally advanced gastric cancer. Transl Oncol 14:100901 (2021). PubMed: 33091827
- Belcher A et al. Targeting Thymidine Phosphorylase With Tipiracil Hydrochloride Attenuates Thrombosis Without Increasing Risk of Bleeding in Mice. Arterioscler Thromb Vasc Biol 41:668-682 (2021). PubMed: 33297751