Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab218381)
Key features and details
- Mouse monoclonal [P-GF.44C] to Thymidine Phosphorylase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Thymidine Phosphorylase antibody [P-GF.44C]
See all Thymidine Phosphorylase primary antibodies -
Description
Mouse monoclonal [P-GF.44C] to Thymidine Phosphorylase -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Thymidine Phosphorylase.
Database link: P19971 -
Positive control
- Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab218381 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
P-GF.44C -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(for 30 minutes at RT) |
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (for 30 minutes at RT) |
Target
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Function
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. -
Pathway
Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. -
Involvement in disease
Mitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. - Information by UniProt
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Database links
- Entrez Gene: 1890 Human
- Entrez Gene: 72962 Mouse
- Entrez Gene: 315219 Rat
- Omim: 131222 Human
- SwissProt: P19971 Human
- SwissProt: Q99N42 Mouse
- SwissProt: Q5FVR2 Rat
- Unigene: 180903 Human
see all -
Alternative names
- ECGF 1 antibody
- ECGF antibody
- ECGF1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab218381 has not yet been referenced specifically in any publications.